soluble cd163 (scd163  (R&D Systems)

Journal: Frontiers in Immunology

Article Title: Identification of common signature genes and pathways underlying the pathogenesis association between nonalcoholic fatty liver disease and heart failure

doi: 10.3389/fimmu.2024.1424308

Figure Lengend Snippet: Genes sorted by support vector machine-recursive feature elimination (SVM-RFE) method.

Article Snippet: Serum soluble CD163 (sCD163) levels were detected using a commercial kit (EM1475; Finetest, Wuhan, China) following the manufacturer’s protocol.

Techniques: Plasmid Preparation

Journal: Frontiers in Immunology

Article Title: Identification of common signature genes and pathways underlying the pathogenesis association between nonalcoholic fatty liver disease and heart failure

doi: 10.3389/fimmu.2024.1424308

Figure Lengend Snippet: Validation and receiver operating characteristic (ROC) curve analyses of CCR1 and CD163 in heart failure (HF) and nonalcoholic fatty liver disease (NAFLD). (A–D) Expression of CCR1 and CD163 in GSE26887 (A) , GSE57345 (B) , GSE126848 (C) , and GSE89632 (D) , respectively. (E–H) ROC curve and area under the curve (AUC) of CCR1 and CD163 in GSE26887 (E) , GSE57345 (F) , GSE126848 (G) , and GSE89632 (H) , respectively. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 vs. healthy controls.

Article Snippet: Serum soluble CD163 (sCD163) levels were detected using a commercial kit (EM1475; Finetest, Wuhan, China) following the manufacturer’s protocol.

Techniques: Expressing

Journal: Frontiers in Immunology

Article Title: Identification of common signature genes and pathways underlying the pathogenesis association between nonalcoholic fatty liver disease and heart failure

doi: 10.3389/fimmu.2024.1424308

Figure Lengend Snippet: Gene Set Enrichment Analysis (GSEA) of CCR1 and CD163 in GSE126848 and GSE26887 datasets. (A, B) Biological processes (A) and KEGG pathways (B) found by singe-gene GSEA of CCR1 in NAFLD dataset GSE126848. (C, D) Biological processes (C) and KEGG pathways (D) found by singe-gene GSEA of CD163 in NAFLD dataset GSE126848. (E, F) Biological processes (E) and KEGG pathways (F) found by singe-gene GSEA of CCR1 in HF dataset GSE26887. (G, H) Biological processes (G) and KEGG pathways (H) found by singe-gene GSEA of CD163 in HF dataset GSE26887.

Article Snippet: Serum soluble CD163 (sCD163) levels were detected using a commercial kit (EM1475; Finetest, Wuhan, China) following the manufacturer’s protocol.

Techniques:

Journal: Frontiers in Immunology

Article Title: Identification of common signature genes and pathways underlying the pathogenesis association between nonalcoholic fatty liver disease and heart failure

doi: 10.3389/fimmu.2024.1424308

Figure Lengend Snippet: Interaction of CCR1 and CD163 with inflammatory genes and chemical drugs. (A) Regulatory network of CCR1 and CD163 and their co-expression genes constructed by the GeneMANIA database. (B, C) The correlation between CCR1 and CD163 with inflammation-related genes in the GSE126848 (B) and GSE26887 (C) datasets. (D) The interactions between CCR1 and CD163 with chemicals and proteins constructed by the STITCH database.

Article Snippet: Serum soluble CD163 (sCD163) levels were detected using a commercial kit (EM1475; Finetest, Wuhan, China) following the manufacturer’s protocol.

Techniques: Expressing, Construct

Journal: Frontiers in Immunology

Article Title: Identification of common signature genes and pathways underlying the pathogenesis association between nonalcoholic fatty liver disease and heart failure

doi: 10.3389/fimmu.2024.1424308

Figure Lengend Snippet: Validation of CCR1 and CD163 in a non-alcoholic fatty liver disease mouse model. (A) Hematoxylin&eosin (H&E) staining of liver tissues in mice with normal chow (10% of calorie from fat, NC) or high-fat diet (60% of calorie from fat, HFD) for 14 weeks. The black arrow indicates infiltrated immune cells. Scale bar = 100 μm. (B) NAFLD activity score (NAS) based on the H&E staining of liver tissues. (C) Hepatic triglyceride (TG) concentrations. (D) Serum alanine aminotransferase (ALT) levels. (E) Relative mRNA expression level of inflammatory marker genes in liver tissues. (F–I) Relative mRNA expression level of Ccr1 (F) , Cd163 (G) , Cd80 (H) , and Cd206 (I) in liver tissues. (J) Representative images under fluorescence microscopy showing CD163 staining (red) and nuclear staining (DIPA, blue) of liver tissues. Scale bar = 100 μm. (K) Representative images under fluorescence microscopy showing CD80 staining (red) and nuclear staining (DIPA, blue) of liver tissues. Scale bar = 100 μm. Mean ± S.E.M., n = 12. * P< 0.05, ** P< 0.01 vs. the NC group.

Article Snippet: Serum soluble CD163 (sCD163) levels were detected using a commercial kit (EM1475; Finetest, Wuhan, China) following the manufacturer’s protocol.

Techniques: Staining, Activity Assay, Expressing, Marker, Fluorescence, Microscopy

Journal: Frontiers in Immunology

Article Title: Identification of common signature genes and pathways underlying the pathogenesis association between nonalcoholic fatty liver disease and heart failure

doi: 10.3389/fimmu.2024.1424308

Figure Lengend Snippet: Validation of CCR1 and CD163 in a heart failure with reduced ejection fraction (HFpEF) mouse model. (A) Wheat germ agglutinin (WGA, green) staining of heart tissues in mice with saline (CON group) or uninephrectomy surgery followed by 0.15mg/h d-aldosterone (HFpEF group) treatment for 4 weeks. Scale bar = 100 μm. (B) Quantitative results of the left ventricular cross-sectional area based on the WGA staining of heart tissues. (C) Heart weight/body weight (HW/BW) ratio. (D–F) Relative mRNA expression level of left ventricular hypertrophy markers Anp (D) , Bnp (E) , and β-MHC (F) in heart tissues. (G) Relative mRNA expression level of inflammatory marker genes in heart tissues. (H–K) Relative mRNA expression level of Ccr1 (H) , Cd163 (I) , Cd80 (J) , and Cd206 (K) in heart tissues. (L) Representative images under fluorescence microscopy showing CD163 staining (red) and nuclear staining (DIPA, blue) of heart tissues. Scale bar = 100 μm. (M) Representative images under fluorescence microscopy showing CD80 staining (red) and nuclear staining (DIPA, blue) of heart tissues. Scale bar = 100 μm. Mean ± S.E.M., n = 6. * P< 0.05, ** P< 0.01 vs. the control group.

Article Snippet: Serum soluble CD163 (sCD163) levels were detected using a commercial kit (EM1475; Finetest, Wuhan, China) following the manufacturer’s protocol.

Techniques: Staining, Saline, Expressing, Marker, Fluorescence, Microscopy, Control

Journal: Frontiers in Immunology

Article Title: Simian immunodeficiency virus-infected rhesus macaques with AIDS co-develop cardiovascular pathology and encephalitis

doi: 10.3389/fimmu.2023.1240946

Figure Lengend Snippet: Animals with CVD pathology and SIVE had increased numbers of cardiac macrophages compared to animals with CVD pathology or SIVE alone, and NSF and SIVnoE animals.

Article Snippet: Plasma IL-18 (R & D Systems; Minneapolis, MN), galectin-3 (R & D Systems), galectin-9 (R & D Systems) and soluble CD163 (sCD163) (IQ Products; Groningen, Netherlands) concentrations were analyzed with ELISAs according to the manufacturer’s protocol.

Techniques:

Journal: Frontiers in Immunology

Article Title: Simian immunodeficiency virus-infected rhesus macaques with AIDS co-develop cardiovascular pathology and encephalitis

doi: 10.3389/fimmu.2023.1240946

Figure Lengend Snippet: Plasma sCD163, IL-18, galectin -3 and -9 are higher in animals with CVD-pathology and SIVE than animals with CVD–pathology or SIVE alone.

Article Snippet: Plasma IL-18 (R & D Systems; Minneapolis, MN), galectin-3 (R & D Systems), galectin-9 (R & D Systems) and soluble CD163 (sCD163) (IQ Products; Groningen, Netherlands) concentrations were analyzed with ELISAs according to the manufacturer’s protocol.

Techniques: Clinical Proteomics

Journal: Frontiers in Immunology

Article Title: Simian immunodeficiency virus-infected rhesus macaques with AIDS co-develop cardiovascular pathology and encephalitis

doi: 10.3389/fimmu.2023.1240946

Figure Lengend Snippet: Plasma sCD163 correlates with galectins- 3 and -9, and IL-18 in SIV infected monkeys. (A-C) . Spearman’s correlation was used to assess the relationship between terminal levels of plasma galectins -3 and -9, and IL-18, and terminal plasma sCD163. (D-G) . Spearman’s correlation was used to assess the relationship between terminal levels of plasma galectins-3 and -9, IL-18, and sCD163, and plasma viral load. Data generated here were from n=23 animals. P-values accepted at Spearman’s p< 0.05.

Article Snippet: Plasma IL-18 (R & D Systems; Minneapolis, MN), galectin-3 (R & D Systems), galectin-9 (R & D Systems) and soluble CD163 (sCD163) (IQ Products; Groningen, Netherlands) concentrations were analyzed with ELISAs according to the manufacturer’s protocol.

Techniques: Clinical Proteomics, Infection, Generated

Journal: Frontiers in Immunology

Article Title: Simian immunodeficiency virus-infected rhesus macaques with AIDS co-develop cardiovascular pathology and encephalitis

doi: 10.3389/fimmu.2023.1240946

Figure Lengend Snippet: Animals with SIVE alone had more plasma sCD163, IL-18, and galectin-3 and -9 than animals with SIVnoE.

Article Snippet: Plasma IL-18 (R & D Systems; Minneapolis, MN), galectin-3 (R & D Systems), galectin-9 (R & D Systems) and soluble CD163 (sCD163) (IQ Products; Groningen, Netherlands) concentrations were analyzed with ELISAs according to the manufacturer’s protocol.

Techniques: Clinical Proteomics

Journal: Frontiers in Immunology

Article Title: Simian immunodeficiency virus-infected rhesus macaques with AIDS co-develop cardiovascular pathology and encephalitis

doi: 10.3389/fimmu.2023.1240946

Figure Lengend Snippet: Individuals with HIVE had greater numbers of cardiac macrophages and collagen deposition than HIVnoE individuals. (A-D) . Increased CD68+, CD163+, and MAC387+ cardiac macrophages in individuals with HIVE. Trend of increased numbers of cardiac CD3+ T lymphocytes regardless of HIVE. Sections of cardiac tissue were stained immunohistochemically with antibodies recognizing CD68+, CD163+, or MAC387+ macrophages, and CD3+ T-lymphocytes. Twenty random, non-overlapping images were sampled at 200x fields of view, and the data was expressed as the average number of cells/mm 2 plus or minus the SEM. (E) . Increased cardiac collagen deposition in individuals with HIVE. Cardiac collagen deposition was assessed using a Masson’s trichrome stain. Fibrosis was determined as the percentage of cardiac collagen per total tissue area and was quantified for using ImageJ Analysis software with twenty random, non-overlapping images sampled at 200x fields of view. Cardiac tissues were supplied by the Manhattan HIV Brain Bank. Data was presented as the average percentage of collagen plus or minus the SEM. P-values were calculated using a nonparametric, Mann-Whitney t test, HIVnoE (circles); HIV with no encephalitis. HIVE (squares); HIV encephalitis ( **** p<0.0001).

Article Snippet: Plasma IL-18 (R & D Systems; Minneapolis, MN), galectin-3 (R & D Systems), galectin-9 (R & D Systems) and soluble CD163 (sCD163) (IQ Products; Groningen, Netherlands) concentrations were analyzed with ELISAs according to the manufacturer’s protocol.

Techniques: Staining, Software, MANN-WHITNEY